Today I submitted a manuscript on a spawning detection method I developed with Drs. Pete Countway and Richard Wahle over the last two years. Unlike my previously published work on spawning using fertilization assays, this methodology depended on environmental DNA (eDNA) and quantitative real-time PCR techniques. In 2016, we had developed our probe and primers for our method, and tested them on diluted sperm in the laboratory. We had to wait until the following summer, however, to test our methodology on naturally spawning sea scallops in the field.
Last summer (2017) we hung nets of sea scallops under the dock at Bigelow Laboratory for Ocean Sciences during the spawning season. We used plankton pumps to take water samples and test our probe and primers for spawning events. Check out my presentation below for the NOAA Central Library Brown Bag Seminar series to preview our results!